Research on the Cellulolytic Activity of Fungi: Pleurotus ostreatus, Pleurotus eryngii and Lentinula edodes Cultivated Using Coffee Grounds and Waste Wood

The objective of the present study was to ascertain the potential of cellulolytic enzymes produced by selected species of basidiomycetes: Pleurotus ostreatus, Pleurotus eryngii, and Lentinula edodes. In the experimental phase, a selection of basidiomycetes were cultivated on waste substrates containing coffee grounds and wood. During the culture, weekly samples of the substrate were taken, from which enzymes were extracted using citrate buffer (BCA) and purified to obtain cellulolytic preparations. The activity of the obtained preparations was then compared with that of commercial cellulase in a hydrolysis reaction of carboxymethylcellulose (CMC). Statistical analysis demonstrated that the preparations obtained from L. edodes (1.785 mg/mg) and P. ostreatus (0.500 mg/mg) cultures exhibited higher activity compared to commercial cellulase (0.041 mg/mg), while preparations from P. eryngii (0.045 mg/mg) demonstrated comparable activity. The findings of this study demonstrate the viability of utilising a waste substrate comprising coffee grounds and wood for the cultivation of basidiomycetes and the production of enzymes.