Plasticization strengthening of the structure of porous biocompatible biodegradable matrices based on polylactoglycolide and its effect on cellular adhesion

Objective. Study of changes in the physico-mechanical and structural characteristics of highly porous matrices based on biocompatible biodegradable polyester — polylactoglycolide. Materials and methods. Samples in the form of a porous polymer fiber were obtained by extrusion of a 10% solution of PLG/TG. The physical and mechanical properties of the material were investigated using a tensile test, the tensile rate was 1 mm/min, the measurement limit was 100% strain or a load of 500 N. The features of the macro- and microstructure of the samples were studied using a stereoscopic optical microscope Bresser Advance (Bresser, Germany) with a digital camera. The hardness measurements of the samples were carried out on a nanohardness meter by instrumental indentation. When studying the adhesion of MMSCs to the surface of matrices, a quantitative assessment of cellular adhesion was performed by calculating at least 15 fields of view in the ImageJ program (NIH, USA). Results. It has been found that as the plasticizer treatment time increases, the strength and modulus of elasticity of the samples increase, but this dependence is not linear. However, along with a significant improvement in physical and mechanical properties, plasticization changes the surface structure of the material, which, in turn, affects cellular adhesion. A decrease in the surface roughness of the samples from 1 micron to 200 nm leads to a decrease in the adhesion of MMSCs on the matrices, compared with untreated samples. Conclusion. Plasticization treatment of polylactoglycolide not only improves the physico-mechanical properties of PLG matrices, but also optimizes their surface for a specific type of cells.