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December 9, 2025Food Additives and Contaminants Part B

Quantification of aflatoxins in animal feed in Punjab, Pakistan, using HPLC-FLD and confirmation by LC-MS/MS

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Authors

MAMateen AbbasAKAbdul Muqeet KhanZHZara Hussain

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Overview

This study quantifies aflatoxins in animal feed in Punjab, indicating high contamination levels pose risks to livestock health.

Key Points

  • To investigate aflatoxin occurrence in animal feed and specific feed ingredients in Punjab, Pakistan.
  • Collected samples from livestock farms in Okara, Punjab (2021-2022)
  • Extracted aflatoxins using acetonitrile-water mixture
  • Purified using Mycosep columns
  • Quantified aflatoxins with HPLC and confirmed by mass spectrometry
  • Aflatoxins detected in 74% of animal feed samples
  • 87% of cottonseed cake and 63% of maize oil cake samples had aflatoxins
  • 33% of animal feed and 70% of oilseed cake samples exceeded the maximum limit of 20 µg/kg
  • Statistically significant results obtained (< 0.05)

Cite This Study

Abbas et al. (2025) studied this question.

synapsesocial.com/papers/69401efa2d562116f28f98bbhttps://doi.org/10.1080/19393210.2025.2576912
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Also Consider

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