Total synthesis achieves 5.5% yield for trisaccharide from Pseudomonas putida, highlighting production challenges and strategies used.
Herein, we report the first total synthesis of the trisaccharide repeating unit of Pseudomonas putida TSh18, achieved through a one-pot glycosylation strategy. The synthesis addresses several challenges, including the synthesis of rare sugars d-Fucp4N₃ and d-QuipNAc4NAc, installation of consecutive 1,2-cis linkages, and a late-stage amide coupling. Access to d-Fucp4N₃ was enabled for the first time by applying a double serial inversion approach. The synthesis was completed in 19 linear steps with an overall yield of 5.5%.
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Behera et al. (2025) studied this question.
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