Ultrafast spectroscopic signatures for off -to- on photoswitchable species in a green-to-red photoconvertible fluorescent protein

Taylor D. Krueger; Cheng Chen; Chong Fang

doi:10.1039/d5sc06279a

December 1, 2025Chemical ScienceOpen Access

Ultrafast spectroscopic signatures for off -to- on photoswitchable species in a green-to-red photoconvertible fluorescent protein

View Full Paper

Ask AI

Bookmark

Share

Key Points

The findings indicate that fluorescent proteins undergo a switch from off to on states via proton transfer.
Ultrafast techniques including Raman spectroscopies were utilized to analyze the transition in fluorescent proteins.
Observation of the photoswitched off state returning to an active state suggests complexity in photoconversion.
These insights highlight potential challenges for applications involving photoconvertible fluorescent proteins.

Implication

Ultrafast analysis reveals fluorescent proteins transition from off to on states via proton transfer, indicating photoconversion challenges.

Abstract

Ultrafast electronic and Raman spectroscopies on least evolved ancestor (LEA) fluorescent proteins reveal a distorted photoswitched off state returning to on state upon photoexcitation via proton transfer and isomerization, hindering photoconversion.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Krueger et al. (2025) studied this question.

synapsesocial.com/papers/69402a8d2d562116f29026be https://doi.org/10.1039/d5sc06279a

View Full Paper

Ask AI

Bookmark

Share

Ultrafast spectroscopic signatures for off -to- on photoswitchable species in a green-to-red photoconvertible fluorescent protein

Key Points

Implication

Abstract

Discussion

Cite This Study

Ultrafast spectroscopic signatures for off -to- on photoswitchable species in a green-to-red photoconvertible fluorescent protein

Key Points

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading