Diagnostic and Promotes Fracture Healing Value of Deregulated miR ‐143‐3p in Patients With Osteoporotic Fractures

Osteoporotic fractures (OPF) impose a significant socioeconomic burden. Therefore, identifying reliable molecular biomarkers for early prediction and precise treatment of OPF is crucial. This study investigated the role of miR‐143‐3p in OPF. We analyzed serum from 188 OPF and 110 osteoporotic (OP) patients. qRT‐PCR and ROC analyses showed that miR‐143‐3p was significantly downregulated in OPF versus OP controls (AUC = 0.893). More importantly, its expression level was markedly higher in patients with normal healing compared to delayed healing, positioning it primarily as a prognostic biomarker for healing outcomes rather than a diagnostic tool for fracture identification. Functionally, miR‐143‐3p inhibited apoptosis, promoted proliferation, and upregulated osteogenic markers ( OCN , OPN , RUNX2 ). Mechanistically, it directly targeted and negatively regulated VASH1, as confirmed by luciferase assays and rescue experiments. In conclusion, miR‐143‐3p may serve as a promising prognostic biomarker for predicting fracture healing outcomes, potentially by targeting VASH1 .