A new species of the genus Gracixalus from Yunnan Wuliangshan National Nature Reserve, China, is described based on morphological and molecular evidence. Morphologically, the new species can be distinguished from other species of the genus by a combination of the following characters: body size relatively large, dorsal surface brownish yellow, dorsal skin rough with dense, small flatten tubercles, tibiotarsal projection absent, heels distinctly overlapping, tibiotarsal articulation reaching anterior corner of eye, and nuptial pad absent in adult male. The genetic distance (uncorrected p-distance) between the new species and other species of the genus ranged from 2.3% to 14.3% in the mitochondrial 16S rRNA gene. This study brings the total number of recognized species within the genus Gracixalus to 24 with 13 of which occur in China and four in Yunnan Province.
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