We report the draft genome sequence of Roseovarius pacificus strain IR27, isolated from anoxic marine sediments on monomethylamine and nitrous oxide. The assembled chromosome is 3,527,725 bp (62.56% GC), and the plasmid is 272,010 bp (59.18% GC). The genome contains denitrification as well as monomethylamine oxidation genes.
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