Immunochromatographic Test System for the Determination of Bisphenol a With Magnetic Concentration

Bisphenol A (BPA) is widely used as a hardener in the production of plastics. However, its release and circulation in ecosystems lead to contamination of drinking water and food products, accompanied by a negative impact on human health, primarily on the endocrine system. In this regard, there is a need for simple and efficient BPA monitoring tools. The paper presents a combination of two approaches for this purpose – the use of magnetic particles (MP) as an antibody carrier and immunochromatographic test strips for recording labeled immune complexes. The visual detection limit of BPA was 10 μg/ml, the instrumental limit was 55 ng/ml, the working range of quantitative determination was 0.1–10 μg/ml. The efficiency of using the MP conjugate for analyte concentration was demonstrated, allowing the detection limit to be reduced by ~200 times. The developed ICA is suitable for qualitative control of the presence and quantitative determination of BPA content in drinking and natural water samples (the detection rate is in the range of 94–106%).