Automated analysis improves insights into histone modifications in single cells, indicating impacts on gene expression and chromatin organization.
Histone post-translational modifications (hPTMs) are central regulators of chromatin organization and gene expression. Their dysregulation is implicated in development, cancer, and aging. While mass spectrometry is the method of choice to study hPTMs, most protocols are designed for bulk samples and cannot resolve cell-to-cell variability. Extending histone PTM analysis to the single-cell level is therefore essential but technically challenging, as histones are low in abundance, lysine-rich, and heavily modified.
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Barotti et al. (2025) studied this question.
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