This study investigates nephrotoxicity induced by beta-cyclodextrin in rats, highlighting renal function decline and involvement of hypoxia-inducible factors.
Key Points
The study examines the nephrotoxic effects of intravenous beta-cyclodextrin and its molecular mechanisms.
Sixteen Wistar rats were used, divided into healthy and diabetic groups.
Both groups received daily intravenous injections of either water or a 6% beta-cyclodextrin solution for 28 days.
Histopathology was analyzed semi-quantitatively on a 0-5 score basis.
RT-PCR quantified the mRNA expression of relevant markers, including HIF-1, HIF-2 and IL-6.
No deaths occurred in any group, but renal function declined in test groups.
Histopathological analysis revealed vascular integrity issues, cellular infiltration, and necrosis.
mRNA expressions of hypoxia-inducible factors were upregulated, indicating regional renal hypoxia.