Three organic soil amendments of different origins (chicken manure, fungal biomass obtained through biological fermentation, and a leonardite-based humic acid product) were applied to young chestnut trees, alongside mineral fertilizer, which when applied alone served as the control. During the second year, bud break pattern, photosynthetic activity, leaf carbohydrate concentrations, soil properties, and leaf nutrient content were evaluated across multiple sampling events. Sampling time significantly influenced most measured parameters. The addition of organic amendments accelerated bud break, influenced plant nutrient uptake, and modified soil properties. Notably, soil organic matter increased following chicken manure and fungal biomass applications, available phosphorus decreased under fungal biomass and leonardite-based humic acids (to 14.5 and 12.4 ppm, respectively, compared to 17.5 ppm in the mineral fertilizer control), and soil iron concentrations tripled under leonardite-based humic acids relative to the control. However, no significant effects were observed on photosynthetic performance or leaf carbohydrate concentrations. Discriminant and hierarchical cluster analyses revealed clear differences among amendments, with the humic acid-based product exerting distinct effects. As there are not many data available in the literature on the efficacy of organic amendments in chestnut cultivation, the present results underscore the importance of the site-specific selection of organic amendments, tailored to soil characteristics (in the present trial, an acidic soil) and specific nutritional objectives to optimize tree physiological performance.
Roussos et al. (Sun,) studied this question.