What question did this study set out to answer?

This research aims to boost tumor immunotherapy by repolarizing macrophages using a probiotic-mediated nanocatalyst.

January 6, 2026Advanced Healthcare Materials0 citations

Biosynthesis of Cu 2‐x Se Nanocatalyst Within Probiotics for Repolarization of Tumor Associated Macrophages to Boost Mild Photothermal Sensitized Chemodynamic Immunotherapy

MWMeichan WuHuazhong University of Science and Technology PLPuze LiHenan Cancer Hospital XXXin XinHuazhong University of Science and Technology

Key Points

This research aims to boost tumor immunotherapy by repolarizing macrophages using a probiotic-mediated nanocatalyst.
Utilized Escherichia coli Nissle 1917 as a bioreactor for biosynthesis of copper selenide nanoparticles.
Characterized the photothermal performance of Cu2-x Se nanoparticles.
Analyzed the activation of the MAPK signaling pathway in macrophages.
Assessed the implications of nanocatalyst-induced hydroxyl radical generation.
Evaluated the effects on dendritic cells and T lymphocyte activation.
Cu2-x Se nanoparticles successfully repolarized macrophages from M2 to M1 phenotype.
Photothermal treatment induced a significant immunogenic death effect in tumor cells.
The Cu2-x Se@EcN formulation led to increased infiltration of T cells in the tumor microenvironment.
Overall, the bacteria-inorganic hybrid demonstrated efficacy in suppressing tumor growth and recurrence.

Abstract

ABSTRACT The activation and infiltration of cytotoxic T lymphocytes (CTLs) are the key of tumor immunotherapy. However, the suppressive tumor immune microenvironment usually inhibits the function of CTLs. Tumor‐associated macrophages (TAMs) play an important role in remodeling the tumor immune microenvironment. In this work, probiotic Escherichia coli Nissle 1917 (EcN) is used as a bioreactor to achieve intracellular synthesis of copper selenide nanoparticles (Cu 2‐x Se NPs), giving Cu 2‐x Se@EcN, which effectively repolarize macrophages from pro‐tumor M2 phenotype to anti‐tumor M1 phenotype mainly through activating MAPK signaling pathway. The synthesized Cu 2‐x Se NPs exhibited excellent photothermal performance, inducing hyperthermia‐sensitized Fenton‐like reaction to catalyze the generation of hydroxyl radical from H 2 O 2 , then resulted in immunogenic death effect of tumor cells and the repolarization of M2 macrophages. Combined with surface‐expressed pathogen‐associated molecular patterns (PAMPs) of bacteria, Cu 2‐x Se@EcN synergistically promoted DCs maturation and TAMs reprogramming, eventually promoting the infiltration and activation of T cells. Therefore, the bacteria‐inorganic hybrid nanomaterials showed satisfied effect in inhibiting tumor growth and recurrence.

Ask AI

Helpful

Bookmark

View Full Paper