A Biocatalytic Cascade‐Based Cu‐GMP Self‐Assembly Nanozyme for Accelerating Infected Diabetic Wound Healing

Nanozymes with multi‐enzyme catalytic properties have emerged as promising multifunctional materials for biosensing and antimicrobial applications. Herein, a copper‐guanosine monophosphate (Cu‐GMP) nanozyme was synthesized via a simple one‐pot method. Its supramolecular network serves as a multifunctional carrier, enabling the encapsulation of functional guest biomolecules and retaining their inherent activity simultaneously. As a proof‐of‐concept, glucose oxidase (GOx) was incorporated to construct a glucose‐responsive cascade catalytic system (GOx@Cu‐GMP). In this system, GOx catalyzes glucose oxidation to produce hydrogen peroxide (H 2 O 2 ) and gluconic acid. The latter creates a localized acidic microenvironment, which combined with in situ generated H 2 O 2 , effectively activates the peroxidase (POD)‐like activity of Cu‐GMP and further generates hydroxyl radicals (•OH). Leveraging this cascade reaction, GOx@Cu‐GMP was developed as a sensitive colorimetric platform for glucose detection. Furthermore, Cu‐GMP exhibits oxidase (OXD)‐, laccase (LAC)‐, and glutathione peroxidase (GPx)‐like activities, facilitating the generation of various reactive oxygen species. Benefiting from multi‑enzyme synergy, it achieves broad‑spectrum antibacterial performance. Moreover, GOx@Cu‐GMP can eliminate bacterial infections and accelerate wound healing in a diabetic mouse skin wound model. This work provides a multifunctional nanozyme‐based platform for glucose detection and diabetic wound antimicrobial therapy, offering new insights for the development of integrated biosensing and therapeutic materials.