miR‐196b‐5p Targets TGFBR3 to Reinforce the Invasion and Migration of LUAD Cells

In this study, we investigated the underlying mechanisms of lung adenocarcinoma (LUAD) by focusing on miR‐196b‐5p as a research target, aiming to provide potential targets for LUAD treatment. Differentially expressed miRNAs and target mRNAs involved in this study were identified through bioinformatics analysis. miR‐196b‐5p and TGF‐β receptor 3 (TGFBR3) mRNA were tested via qRT‐PCR. Western blot (WB) analysis was undertaken to test the expression of TGFBR3 protein. Cell scratch‐healing and transwell assays were employed to examine the invasive and migratory abilities of LUAD cells. Furthermore, the interaction between miR‐196b‐5p and its downstream target genes was confirmed through a dual luciferase assay. The results showed that miR‐196b‐5p was upregulated in LUAD cells, while TGFBR3 expression was significantly downregulated. High expression of miR‐196b‐5p facilitated the migratory and invasive capabilities of LUAD cells. Furthermore, dual‐luciferase assays validated that TGFBR3 was a specific target of miR‐196b‐5p in LUAD cells. miR‐196b‐5p in LUAD cells targeted to downregulate TGFBR3 to enhance cell invasion and migration. In summary, our study reveals that miR‐196b‐5p targets and downregulates TGFBR3, thereby boosting the migration and invasion of LUAD cells.