What question did this study set out to answer?

This research aims to explore how omega-3 polyunsaturated fatty acids modulate inflammatory pathways in cardiac mature adipocytes and stem cells.

January 10, 2026Food & Function0 citationsOpen Access

Omega-3 PUFAs Reduce Inflammation by Targeting NRF2 and NF-κB activity in an Ex-Vivo Model of Cardiac mature Adipocytes and adipose derived stem cells from Atherosclerotic Patients

Q: What does this research mean for the field?

Omega-3 polyunsaturated fatty acids reduce inflammatory activity in cardiac mature adipocytes and stem cells from atherosclerotic patients by targeting NRF2 and NF-κB pathways. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

SQStefano QuartaUniversity of Salento GSGIUSEPPE SANTARPINOMagna Graecia University NCNadia CalabrisoIstituto di Fisiologia Clinica

Key Result

Omega-3 polyunsaturated fatty acids reduced inflammatory activity in cardiac mature adipocytes and stem cells from atherosclerotic patients by targeting NRF2 and NF-κB pathways.

Key Points

This research aims to explore how omega-3 polyunsaturated fatty acids modulate inflammatory pathways in cardiac mature adipocytes and stem cells.
Utilized an ex-vivo model with cardiac mature adipocytes and adipose-derived stem cells.
Investigated the effects of omega-3 PUFAs on NRF2 and NF-κB activities.
Analyzed inflammatory markers and pathways related to atherosclerosis.
Omega-3 PUFAs significantly reduced the activity of NF-κB, a key inflammatory regulator.
NRF2 activity increased, indicating a protective antioxidant response.
A reduction in pro-inflammatory cytokines was observed in the treated adipocytes and stem cells.

Structured PICO

Does ex-vivo treatment with DHA and EPA reduce pro-inflammatory gene expression and activity in cardiac adipocytes from CAD patients?

Population

Ex-vivo model of cardiac mature adipocytes and adipose-derived stem cells (ASCs) isolated from pericardial adipose tissue (PAT) of 12 male patients (mean age 69.7) undergoing coronary artery bypass graft surgery (CABG).

Intervention

Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) at 50 µmol L−1 for 48 hours.

Comparator

Untreated control cells and cells treated with arachidonic acid (AA).

Outcome

Expression and secretion of pro-inflammatory mediators (MCP-1, IL-6, CXCL10) and lipid mediators (RvD1) at 48 hours.surrogate

DHA and EPA attenuate the inflammatory profile of cardiac adipocytes from CAD patients via NRF2 activation and NF-κB suppression, highlighting a potential mechanism for their atheroprotective effects.

Main Result

Absolute Event Rate: 0% vs 0%

Abstract

Under proatherogenic conditions, epicardial (EAT) and pericardial adipose tissue (PAT) acquire inflammatory/pro-atherogenic phenotypes that contribute to coronary atherosclerosis. Recent data have highlighted a significant inverse relationship between levels of n-3...

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