The novel spectrophotometric method for quantifying paclitaxel showed linearity from 1.0–55 μg/mL, with LOD of 0.0098 μg/mL and recovery values between 98.70%–100.23%.
A novel, direct, and reliable spectrophotometric method using osmium tetroxide was developed and validated for the accurate quantification of paclitaxel in pharmaceutical formulations and biological fluids.
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Paclitaxel (Pac) is an important anticancer bioactive compound, and the availability of an accurate and robust analytical method for its quantification is of utmost importance. In this investigation, a novel, direct, and reliable spectrophotometric method has been proposed for the estimation of Pac via a complex formation reaction with osmium (Os). The method was based on the reaction of Pac with Os tetroxide, which produced a yellowish-brown complex. The resulting complex is soluble in water and displays a maximum absorption peak at 482 nm. The reaction conditions, including reactant concentration, pH, temperature, and reaction time, were optimized to obtain the best spectrophotometric response. A thorough method validation study was conducted, demonstrating that the calibration curve is linear over the concentration range of 1.0–55 μg/mL and follows Beer’s law, with a molar absorptivity of 3.01 × 10⁻4 L/mol⁻1.cm⁻1. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.0098 and 0.0328 μg/mL, respectively. The recovery values were planned and found to be 98.70%–100.23%. At the same time, the precision values (represented by relative standard deviation percent RSD%) were better than 0.81% (in injections) and 1.859% (in biological fluids), depending on the Pac concentration. The suggested method was successfully applied to estimate Pac in a pharmaceutical formulation (as injections) and in spiked biological fluids (serum and urine), with results showing good accuracy and precision compared to the reference method.
A.A.SALEEM et al. (Thu,) reported a other. The novel spectrophotometric method for quantifying paclitaxel showed linearity from 1.0–55 μg/mL, with LOD of 0.0098 μg/mL and recovery values between 98.70%–100.23%.