Key result
A rapid and sensitive LC-MS/MS method using chloroform extraction was developed and validated for determining paclitaxel in human plasma, demonstrating a lower limit of detection of 10 pg/mL.
A rapid and sensitive LC-MS/MS method was successfully developed and validated for the determination of paclitaxel in human plasma, which is useful for pharmacokinetic analysis.
New validated LC-MS/MS assay enables precise paclitaxel plasma quantification; leaves open cardiovascular pharmacokinetic applications pending prospective validation.
A rapid and sensitive liquid chromatography -tandem mass spectrometry (LC-MS/MS) method for determination of the Paclitaxel in human plasma was developed and validated.Placitaxel was extracted from plasma by a two-step extraction procedure with using of chloroform as Liquid -Liquid extractive organic solvent.LC-MS/MS analysis using electro-spray ionization (ESI+) was performed on Phenomenex C-18 Column (250x1.5μ)using as acetonitrile, water (80:20+0.1%acetic acid) as mobile phase.The method has a fl ow rate of 0.8ml/min.Retention of Paclitaxel was 4.60 minutes.An excellent linearity (r 2 .099)between the peak ratios and Paclitaxel concentrations over the range of 10-100 ng/ml of plasma was studied.The lower limit of detection for Paclitaxel on mass was 10 pg/mL.There was about 100 percent of Paclitaxel was recovered in extracted samples.The study of Paclitaxel standard and extraction standard calibration curves were useful in pharmacokinetics analysis.
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Rajender et al. (2010) studied Healthy volunteers (plasma samples). LC-MS/MS method with chloroform extraction was evaluated on Method validation (linearity, recovery, accuracy, precision). A rapid and sensitive LC-MS/MS method using chloroform extraction was developed and validated for determining paclitaxel in human plasma, demonstrating a lower limit of detection of 10 pg/mL.
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