P-1534. Development of PCRSeqTyping to Differentiate Streptococcus pneumoniae (SP) Serotypes 15B and 15C

Ilan Rozen Eisenberg; Yazdani B. Shaik-Dasthagirisaheb; Stephen I. Pelton

doi:10.1093/ofid/ofaf695.1715

January 1, 2026Open Forum Infectious DiseasesOpen Access

P-1534. Development of PCRSeqTyping to Differentiate Streptococcus pneumoniae (SP) Serotypes 15B and 15C

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Key Points

This research aims to develop a rapid and accurate method to differentiate between Streptococcus pneumoniae serotypes 15B and 15C.
DNA extraction performed on SP negative samples
QRT-PCR used with LytA and PiaB genes to confirm SP
Molecular serotyping with serotype specific primers and probes
Amplification of samples using O-Acetyl Transferase gene specific primers for further analysis
Sequencing of PCR DNA for confirmation
100% correlation of PCRSeqTyping results with sequencing results of positive controls
Eight culture negative samples accurately identified as either 15B (n=4) or 15C (n=4)

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Implication

PCRSeqTyping shows accurate differentiation of Streptococcus pneumoniae serotypes 15B and 15C in culture negative samples, suggesting improved surveillance for invasive pneumococcal disease.

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Cite This Study

Eisenberg et al. (2026) studied this question.

synapsesocial.com/papers/6966f2f013bf7a6f02c0055e https://doi.org/10.1093/ofid/ofaf695.1715

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P-1534. Development of PCRSeqTyping to Differentiate Streptococcus pneumoniae (SP) Serotypes 15B and 15C

Key Points

No abstract yet

Implication

Discussion

Cite This Study

P-1534. Development of PCRSeqTyping to Differentiate Streptococcus pneumoniae (SP) Serotypes 15B and 15C

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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