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January 14, 2026Foods0 citationsOpen Access

Effect of Different Preservation Methods on the Microbiological Quality, Physicochemical, and Antioxidant Properties of Red Prickly Pear (Opuntia streptacantha) Juice

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JJJorge Alberto Jose-SalazarDHDiana Maylet Hernández-MartínezECEliseo Cristiani‐Urbina

Key Points

  • The study aims to determine the best preservation methods for maintaining quality in red prickly pear juice during storage.
  • Investigated four preservation techniques: refrigeration, freezing, pasteurization, and vacuum packaging.
  • Assessed microbiological quality, physicochemical properties, and antioxidant capacity of the juice.
  • Conducted sensory analysis to compare preservation effectiveness of treated juice against control.
  • Freezing and vacuum packaging with pasteurization (method J4) was most effective for preserving juice quality.
  • After 12 months, J4 maintained microbial growth reduction and key qualities like pH (4.64) and acidity (0.74 g citric acid L–1).
  • Antioxidant activity (2.6–2.9 mmol TE L–1) and phenol content (506 mg GAE L–1) were also preserved, along with betalains (141.2 mg L–1) and total sugars (125 g L–1).
  • No significant sensory differences were found between J4-treated and control juice.

Abstract

The red prickly pear (Opuntia streptacantha) is a fruit that is distinguished by its sensory properties and high content of bioactive compounds. Its rapid spoilage rate significantly impacts its commercialization, underscoring the urgent need for effective preservation methods. This study investigated the effectiveness of various juice preservation techniques—refrigeration, freezing, pasteurization, and vacuum packaging —in maintaining the microbiological quality, physicochemical properties, and antioxidant capacity of red prickly pear juice during storage. The most effective preservation method was found to be freezing the vacuum-packaged and pasteurized juice, referred to as J4. This method adequately maintained key nutritional and physicochemical qualities after 12 months, which was evidenced by a reduction in the microbial growth and the preservation of pH (4.64), acidity (0.74 g citric acid L–1), antioxidant activity (2.6–2.9 mmol TE L–1), as well as the content of phenols (506 mg GAE L–1), betalains (141.2 mg L–1), and total sugars (125 g L–1). Furthermore, sensory analysis comparing J4-treated juice to control juice revealed no significant differences, confirming that J4 is an effective method for preserving the nutritional, functional, and sensory qualities of red prickly pear juice.

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Cite This Study

Jose-Salazar et al. (2026) studied this question.

synapsesocial.com/papers/6967191987ba607552bb90d4https://doi.org/10.3390/foods15020282
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