Spatial organization of an enzyme cascade in Ni-ZIF-8 framework for efficient sugar nucleotide synthesis

Key Points

• The aim is to improve the catalytic efficiency of enzyme cascade reactions for sugar nucleotide biosynthesis.
• Utilization of Ni-ZIF-8 framework for enzyme immobilization
• Assessment of enzyme stability and activity
• Evaluation of intermediate utilization during the cascade
• Demonstrated increased catalytic efficiency compared to unstructured systems
• Showed improved stability of individual enzymes in the framework
• Highlighted effective utilization of intermediates during the synthesis process