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The research aims to enhance gene knockout efficiency and specificity using a multiplexed Cas12a system.

January 17, 2026Nature CommunicationsOpen Access

Improved in vivo gene knockout with high specificity using multiplexed Cas12a sgRNAs

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The research aims to enhance gene knockout efficiency and specificity using a multiplexed Cas12a system.
Developed a Cas12a-based system targeting genes with four sgRNAs each.
Assessed Cas12a activity across a large portion of the Drosophila genome.
Compared performance to existing Cas9-based systems targeting over 100 genes.
Achieved greater than 99% on-target activity with less than 1% off-target activity.
Demonstrated superior performance compared to current gene editing methods.
Revealed new phenotypes not detected by established methods.

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Demonstrates improved gene knockout in Drosophila using multiplexed CRISPR-Cas12a sgRNAs, indicating high specificity and efficacy.

Port et al. (2026) studied this question.

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