What question did this study set out to answer?

The research aims to uncover the molecular mechanism by which gibberellin enhances sugar accumulation in longan fruit.

January 18, 2026Open Access

Gibberellin Promotes Sugar Accumulation in Longan Fruit via Upregulation of the Plasma Membrane Sugar Transporter DlSWEET3a

TXTao XieFujian Agriculture and Forestry University YBYuying BaoInner Mongolia University JXJinglei XuAll-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery

Key Points

The research aims to uncover the molecular mechanism by which gibberellin enhances sugar accumulation in longan fruit.
Administered gibberellin (GA3) at 50 mg/L to longan fruit.
Conducted transcriptomic analysis to identify differentially expressed genes.
Confirmed subcellular localization of DlSWEET3a on the plasma membrane.
Performed functional assays in yeast to test sugar transport capabilities.
Executed transient and stable overexpression of DlSWEET3a in longan fruit and tobacco leaves.
GA3 significantly increased sucrose, glucose, fructose, and total sugar content in longan.
Identified 1345 differentially expressed genes, including upregulation of DlSWEET3a.
DlSWEET3a effectively transported glucose, fructose, mannose, and galactose in yeast.
Overexpressing DlSWEET3a in longan and tobacco resulted in higher soluble sugar content.

Abstract

Exogenous gibberellin (GA3) significantly improves sugar accumulation in longan (Dimocarpus longan) fruit, yet its molecular mechanism remains unclear. This study demonstrates that 50 mg/L GA3 optimally enhances sucrose, glucose, fructose, total sugar, and sweetness in longan. Transcriptomic analysis revealed 1345 differentially expressed genes (DEGs), including the sugar transporter gene DlSWEET3a, which was upregulated by GA3. Subcellular localization confirmed DlSWEET3a resides on the plasma membrane. Functional assays in yeast demonstrated its ability to transport glucose, fructose, mannose, and galactose. Critically, transient overexpression of DlSWEET3a in longan fruit and stable overexpression in tobacco leaves significantly increased soluble sugar content. These results establish that GA3 promotes sugar accumulation in longan fruit partly through the upregulation of the plasma membrane hexose transporter DlSWEET3a, providing a mechanistic insight into gibberellin-mediated fruit quality improvement.

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