Assessment of solvent exposure of native cysteines in human Hsp90 using thiol-reactive functional tags

Key Points

• The aim is to examine the solvent exposure of native cysteines in human Hsp90 to understand their role in protein dynamics.
• Utilized thiol-reactive functional tags to study cysteines in Hsp90.
• Conducted experiments under physiological solution-phase conditions.
• Analyzed solvent exposure using biochemical assays.
• Identified specific cysteines with significant solvent exposure.
• Demonstrated that cysteine exposure varies with conformational states of Hsp90.
• Provided insights into the chaperone's structural dynamics under physiological conditions.