Between 2022 and 2024, a severe branch dieback disease was observed affecting over 6% of sweet cherry trees of the ‘Tieton’ cultivar in commercial greenhouses in southern Liaoning Province, China. Symptoms primarily occurred at the top of young branches. At the early stage of disease onset, the lesions appeared as dark brown, irregularly shaped areas with a moist surface; as the disease progressed, these lesions turned dry and rotten, leading to tree decline symptoms in sweet cherry trees. Disease diagnosis was carried out in sweet cherry greenhouses across Liaoning Province, where 24 diseased samples were collected and 14 fungal isolates were obtained therefrom. Based on morphological traits, cultural characteristics, and multi-locus phylogenetic analyses of the internal transcribed spacer (ITS) region, beta-tubulin (TUB2) gene, and translation elongation factor 1-α (TEF1) gene, these isolates were identified as Botryosphaeria dothidea. Two representative isolates, namely zdcy-1 and zdcy-2, were selected for pathogenicity assays. Both mycelial plug and spore suspension inoculation methods confirmed the pathogenicity of the pathogen. The biological characteristic assays revealed that the optimal temperature range for the pathogen’s mycelial growth on PDA medium was 25–28 °C, and the optimal pH range was 6.0–8.0. This study improves the understanding of branch dieback disease in sweet cherry orchards in China, enriches the knowledge regarding the geographical distribution, host range, and infection sites of the pathogen, and provides novel insights for the management of sweet cherry diseases.
Dai et al. (Mon,) studied this question.