ABSTRACT Purpose: An Innovative and economical method of “Microscopic Photo-quilting” to create a virtual slide for teaching and learning. Materials and Methods: Capturing Individual focal images with a Digital Research Microscope using “High Frequency Wave Pattern” and digitally quilting them to create a seamless virtual image. Results: Microscopic Photo-quilting can be used to create a virtual seamless image/virtual slide economically and manually using easy to follow steps and without the need for huge investments for procuring whole slide imaging scanners. Conclusion: Virtual slides can be implemented as a virtual and practical teaching tool for both the undergraduates and post-graduate students. These slides can serve as an excellent reference tool for students as individual and multiple areas of interest can be highlighted upon the virtual slide itself. The virtual slide serves as a Whole slide interface as the user can focus on any area of interest without distortion and the need to change objectives.
Pattnaik et al. (Mon,) studied this question.