Abstract Background: Our understanding of the mechanisms of tumor immunosuppression in the tumor microenvironment (TME) of aggressive prostatic adenocarcinoma (PCa) at the single-cell level is limited. Integrated Visium-HD Spatial RNAseq analysis and 10X Flex sequencing of formalin-fixed paraffin-embedded (FFPE) tissue can provide unique insights into the mechanisms of immunosuppression in the ‘immune cold’ TME of PCa. Design: Visium-HD Spatial RNAseq analysis was performed on FFPE blocks from radical prostatectomy specimens of 12 patients of which six had lymph node metastases (LNM) and six did not. All specimens were also analyzed by 10X Flex sequencing, and data from each scRNAseq dataset were integrated with spatial RNAseq data. VisiumHD bins were processed with H 2026 Jan 20-22; Philadelphia PA. Philadelphia (PA): AACR; Cancer Res 2026;86 (2Suppl): Abstract nr A046.
Ombor et al. (2026) studied this question.