Fusarium wilt (FW), caused by Fusarium oxysporum, poses a significant threat to mungbean (Vigna radiata L.), impacting its yield and quality. In this study, a recombinant inbred line (RIL) population was developed by crossing the highly resistant cultivar Weilv 9002-341 with the highly susceptible line V1128. Assessment of resistance revealed a continuous variation in the average disease index within the resulting population, consistent with the inheritance pattern of quantitative traits. Leveraging an F2:3 segregating population, we conducted linkage mapping analysis and bulked segregant analysis by sequencing, leading to the construction of a genetic linkage map and the identification of a region correlated with resistance. Within this region, 14 novel simple sequence repeat markers were designed to enable refined mapping. A putative resistance locus, spanning 0.17 Mb and encompassing 19 annotated genes, was precisely located. Ultimately, two genes were identified as high-priority candidates conferring resistance. The results of this study lay the foundation for the functional investigation of genes associated with resistance to Fusarium wilt disease in mungbean.
Shen et al. (Tue,) studied this question.