What question did this study set out to answer?

The research aims to investigate how IL-22 influences inflammatory pathways and drug resistance in ulcerative colitis through a JAK1-dependent mechanism.

January 23, 2026Journal of Crohn s and Colitis

P0179IL-22-driven inflammatory and drug resistance pathways are JAK1-dependent in a human 3D mini-gut organoid system

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The research aims to investigate how IL-22 influences inflammatory pathways and drug resistance in ulcerative colitis through a JAK1-dependent mechanism.
Isolated colonic crypts from inactive ulcerative colitis and healthy control patients.
Developed organoids and differentiated them in Wnt-free media for 72 hours.
Examined the effect of upadacitinib and IL-22 on gene expression and protein levels.
Performed RNA extraction and profiling using Next Generation Sequencing.
Analyzed differential gene expression and pathways with statistical software.
IL-22 stimulation elicited a significant transcriptional response in organoids, with 4900 DEGs identified from ulcerative colitis samples.
Upadacitinib inhibited key proinflammatory transcripts dose-dependently, notably metalloproteinases.
Increased transcriptional changes were observed in ulcerative colitis organoids compared to healthy controls.

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Implication

Demonstrates IL-22-driven drug resistance in ulcerative colitis, highlighting JAK1's role in epithelial cells.

Cite This Study

Saifuddin et al. (2026) studied this question.

synapsesocial.com/papers/69730eabc8125b09b0d1e7d0 https://doi.org/10.1093/ecco-jcc/jjaf231.360

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P0179IL-22-driven inflammatory and drug resistance pathways are JAK1-dependent in a human 3D mini-gut organoid system

Key Points

Discussion

Implication

Cite This Study

P0179IL-22-driven inflammatory and drug resistance pathways are JAK1-dependent in a human 3D mini-gut organoid system

Key Points

Discussion

Implication

Cite This Study

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