Marsh beetles (Coleoptera: Scirtidae) of the Amazon biome: integrative collecting techniques, information of immatures' habitats, and a rearing method under laboratory conditions, along with new species records.

Scirtidae are beetles whose larvae are aquatic and can be found in various habitats. The objectives of this study were to explore several aquatic habitats in the Amazon biome in search of Scirtidae using different methods for collecting larvae and adults, to propose a new reproduction method, and to report new records. Eight aquatic habitats were sampled: temporary ponds, streams, lakes, rivers, and four types of phytotelmata, including bromeliads, wild banana trees (Strelitziaceae), bracts, and palm sheaths (Arecaceae). These surveys were conducted across six states of the Brazilian Amazon: Acre, Amapá, Amazonas, Pará, Rondônia, and Roraima. A total of 1,125 larvae were collected, of which 423 successfully developed into adults through laboratory rearing. In addition, 503 adult specimens were collected by sweeping vegetation and using traps. We also developed a laboratory rearing method capable of monitoring several larvae simultaneously to obtain adult specimens and biological information on immatures. As a result, four genera were recorded: Contacyphon, Ora, Scirtes and a new unnamed genus. The genus Contacyphon and the species Ora atroapicalis Pic, O. bivittata Pic, and O. depressa (Fabricius) were recorded for the first time in the Brazilian Amazon. Rearing allowed us to observe four different types of pupation.