What question did this study set out to answer?

To develop a reliable analytical method for profiling phospholipids in lecithin used as an emulsifier in food supplements.

January 23, 2026SeparationsOpen Access

Phospholipid Profiling: A Computationally Assisted LC-HRMS Approach in Lecithin

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This analytical study evaluates emulsification properties in lecithin using a new LC-HRMS method, highlighting its implications for food supplements.

To develop a reliable analytical method for profiling phospholipids in lecithin used as an emulsifier in food supplements.
Developed liquid chromatography–mass spectrometry method for phospholipid identification
Optimized reverse-phase high-performance liquid chromatography using a specific column
Used a custom Python program for generating phospholipid species lists based on mass-to-charge ratios
Confirmed phospholipid identities via tandem mass spectrometry and comparison with standard compounds
Demonstrated good linearity and precision in chromatography
Identified various phospholipid species without standard reference materials
Validated approach with commercially available standard compounds
Provided a cost-effective strategy for lipid profiling in complex matrices

Šijanec et al. (2026) studied this question.

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This analytical study evaluates emulsification properties in lecithin using a new LC-HRMS method, highlighting its implications for food supplements.