What question did this study set out to answer?

This research aims to develop a viability-informed single-cell mass spectrometry technique to enhance metabolic profiling.

January 25, 2026Analytical Chemistry

Viability-Informed Single-Cell Mass Spectrometry for More Comprehensive Metabolic Analysis

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This research aims to develop a viability-informed single-cell mass spectrometry technique to enhance metabolic profiling.
Developed a high-throughput, label-free single-cell mass spectrometry technique (ViSCMS).
Used phosphocholine (PC) 34:1 as a signal for confirming cell viability and metabolic events.
Identified glutathione (GSH) as a marker for distinguishing live from dead cells.
Compared viability rates with conventional AO/PI staining.
Subtyped colorectal cancer (CRC) cell lines based on viability data.
ViSCMS showed a mean bias of 0.33% compared to conventional viability methods.
Achieved excellent reproducibility across different cell lines (SD < 3%).
Successful subtyping of six colorectal cancer cell lines based on cell viability.
Observed clear metabolic differences in HCT116 cells treated with an anticancer drug.

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Implication

Viability-informed mass spectrometry assesses metabolic profiles in colorectal cancer cells, suggesting significant insights into cancer therapy adaptation.

Cite This Study

Cheng et al. (2026) studied this question.

synapsesocial.com/papers/6975b1cefeba4585c2d6d46b https://doi.org/10.1021/acs.analchem.5c07083

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Implication

Viability-informed mass spectrometry assesses metabolic profiles in colorectal cancer cells, suggesting significant insights into cancer therapy adaptation.

Viability-Informed Single-Cell Mass Spectrometry for More Comprehensive Metabolic Analysis

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Discussion

Implication

Cite This Study

Viability-Informed Single-Cell Mass Spectrometry for More Comprehensive Metabolic Analysis

Key Points

Discussion

Implication

Cite This Study

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