Introduction:: One of the most important materials needed for root canal therapy is a sealer. The aim of this study was to assess the tissue reaction of a new polycaprolactone-based root canal sealer in a rat model. method: Eighty adult male rats were randomly divided into 5 groups (n=16, for assessment at 3, 7, 15, 30, and 60 days' intervals). The rats were anesthetized with Ketamine and Xylazine. Surgical anesthesia lasted thirty minutes. After shaving their back hair and cleaning the spot with 5% iodine, two grooves with a length of 1 cm were created with a scalpel no. 15 Thus, one of the tested materials was randomly inserted into each of the incisions, and an empty polyethylene angioket tube with a length of 8 mm and an internal diameter of 1.1 mm was inserted into one of the incision sites as a control. After the 3, 7, 15, 30, and 60 day intervals, the rats were anesthetized again. The rats were sacrificed by overdosing on an anesthetic drug injection (barbiturate or ether). The samples were kept in 10% formalin and then sent for slide preparation. After preparing the paraffin blocks, longitudinal sections were prepared along the axis of the tubes with a thickness of 6 μm, passing through the studied material. Four sections were prepared from each block, passing longitudinally through the largest diameter of the tube. result: The results showed that there was no significant difference between the case and control groups in the degree of inflammation and the cell count (lymphocytes, macrophages, mast cells, plasma cells, neutrophils, eosinophils) in the study groups during the two-month evaluation period (P-value < 0.05). Discussion:: These results support its potential for clinical application, although further studies are needed to confirm safety and efficacy in human treatments. Conclusion:: The studied new sealer was biocompatible in the animal rat model.-
Attari et al. (Thu,) studied this question.