What question did this study set out to answer?

The aim is to enhance genome editing efficiency in rice and tomato using a new optimized platform.

February 2, 2026Open Access

A surrogate‐selection‐enhanced TnpBmax platform enables efficient genome editing in rice and tomato

ELE. F. LiChinese Academy of Agricultural Sciences JXJiyong XieRongcheng City People's Hospital XLXinbo LiChinese Academy of Agricultural Sciences

Key Points

The aim is to enhance genome editing efficiency in rice and tomato using a new optimized platform.
Utilized TnpB, a RNA-guided nuclease, optimized for plant genome editing
Developed TnpBe5 by fusing enhanced RNA with T5 exonuclease
Coupled TnpBmax with a HYYG surrogate for enriched cell selection
Achieved a 2.5-fold increase in editing efficiency in rice
Reached up to 81.5% editing efficiency in selected cells
Ensured high homozygosity in genome-edited rice and tomato plants

Abstract

TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrogate (TnpBmax) enriches edited cells, achieving up to 81.5% efficiency and high homozygosity across rice and tomato.

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