From Two to One: Resolving CO Binding in Acetyl-CoA Synthase

Key Points

• This research investigates the binding dynamics of carbon monoxide in acetyl-CoA synthase.
• Conducted low-temperature ENDOR studies to analyze CO binding
• Investigated the catalytic process of acetyl-CoA formation
• Monitored interaction between substrates and the enzyme
• Identified key interactions between carbon monoxide and acetyl-CoA synthase
• Demonstrated resolved binding pathways for CO
• Highlighted implications for the understanding of biomolecular fixation processes