Ancestral Sequence Reconstruction Reveals New Functional Fluorinases and Mechanistic Insights into Enzymatic Fluorination

Shreyas Supekar; Wan Lin Yeo; Elaine Tiong; Juliana Rizal; Ee Lui Ang; Fong Tian Wong; Yee Hwee Lim; Hao Fan

doi:10.1039/d5cc06378g

January 1, 2026Chemical Communications

Ancestral Sequence Reconstruction Reveals New Functional Fluorinases and Mechanistic Insights into Enzymatic Fluorination

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Key Points

The research aims to expand the diversity of fluorinases, enzymes that form carbon-fluorine bonds, using ancestral sequence reconstruction.
Utilized ancestral sequence reconstruction techniques
Identified and characterized seven functional fluorinases
Conducted comparative analysis to determine enzyme activity
Discovered seven new functional fluorinases with 28-79% conversion rates
Expanded understanding of fluorinase enzyme diversity
Provided insights into the mechanisms of enzymatic fluorination

Implication

Ancestral reconstruction reveals new fluorinases, enhancing understanding of enzyme function and diversity.

Abstract

Fluorinases catalyze carbon-fluorine bond formation and represent a rare but valuable enzyme class. We employed ancestral sequence reconstruction to expand fluorinase diversity, identifying seven functional enzymes (28-79% conversion). Comparative analysis...

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Cite This Study

Supekar et al. (2026) studied this question.

synapsesocial.com/papers/6985859b8f7c464f23009138 https://doi.org/10.1039/d5cc06378g

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Ancestral Sequence Reconstruction Reveals New Functional Fluorinases and Mechanistic Insights into Enzymatic Fluorination

Key Points

Implication

Abstract

Discussion

Cite This Study

Ancestral Sequence Reconstruction Reveals New Functional Fluorinases and Mechanistic Insights into Enzymatic Fluorination

Key Points

Implication

Abstract

Discussion

Cite This Study

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