Stem nitrogen accumulation through vegetative storage proteins and mobilization to seeds supports high-yielding soybean

Abstract The role of stems as a source of nitrogen (N) for the growing seeds has received little attention in soybean. This study evaluated N storage and mobilization from stems to seeds across 42 high-yielding environments under contrasting N supply: a “zero-N” treatment, in which crops relied on soil and biological N fixation, and a “full-N” treatment that received ample N fertilizer. We measured the accumulated N per organ before seed filling and subsequent N mobilization from vegetative organs to the seed. Vegetative storage protein (VSP) abundance in stems was quantified via proteomics. Average yield ranged from 4.2 to 7.3 Mg ha-1, with +11% yield in full-N (6.1 Mg ha-1) compared to zero-N (5.5 Mg ha-1). The full-N treatment resulted in a larger mobilized N from stems (+42%) and leaves (+22%) to seeds than the zero-N treatment, which was attributed to the larger N stored in stems and leaves before seed filling (+37% and +21%, respectively). Among the 5,335 identified proteins at the peak of N accumulation, VSPα (+1.4%) and VSPβ (+0.8%) showed the highest increase in full-N. We conclude that N stored in stems as VSPs before seed filling plays a key role in meeting the seed N demand in high-yielding soybean crops.