Development and validation of a sample-to-result real time multiplex RT-PCR test for human African trypanosomiasis and arboviral febrile illnesses in the Democratic Republic of the Congo | Synapse
February 8, 2026Open Access
Development and validation of a sample-to-result real time multiplex RT-PCR test for human African trypanosomiasis and arboviral febrile illnesses in the Democratic Republic of the Congo
Key Points
To develop and validate a rapid diagnostic test for human African trypanosomiasis and arboviral febrile illnesses.
Created a real-time multiplex RT-PCR test.
Tested the diagnostic accuracy in samples from affected individuals.
Registered trial on ClinicalTrials.gov.
The multiplex RT-PCR test demonstrated high sensitivity and specificity.
Successful detection of both human African trypanosomiasis and arboviral infections was achieved.
Abstract
Clinicaltrials.gov NCT04760678, registered on February 17th, 2021.