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The study aims to identify WOX gene family members in Euphorbia hirta and explore their roles in stem development.

February 9, 2026PlantsOpen Access

Genome-Wide Identification and Expression Analysis of the WOX Family Reveals Potential Roles in Stem Development of Euphorbia hirta

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The study aims to identify WOX gene family members in Euphorbia hirta and explore their roles in stem development.
Identified 14 EhWOX genes in Euphorbia hirta
Characterized their properties, locations, and expression patterns
Conducted phylogenetic analysis to classify genes into clades
Performed RNA-seq and RT-qPCR to confirm expression levels
Analyzed promoter cis-elements for regulatory functions
Identified 14 EhWOX genes distributed across nine chromosomes
High expression levels of EhWOX4-6 and EhWOX14 in stems confirmed by RNA-seq and RT-qPCR
Promoter analyses revealed light, hormone, and stress-responsive elements
Phylogenetic analysis categorized genes into three clades
Some WOX proteins showed potential for autoactivation and localization in stem tissues

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This research identifies and analyzes WOX genes in Euphorbia hirta, highlighting their roles in stem development and laticifer biology.

Lyu et al. (2026) studied this question.

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