De novo transcriptome assembly reveals high-quality gene expression in silver fir saplings, indicating its genomic potential.
The silver fir, Abies alba Mill., is a key species in European mountain forest ecosystems, which, despite being one of the few conifers with an available reference genome, lacked high quality transcriptomic information. Here, we present a de novo transcriptome assembly of this species, obtained using both short- and long-read sequencing data, from three different organs: leaves, stem, and roots. Saplings were subjected to different treatments, including cold, heat, drought, and darkness, in order to induce the expression of as many genes as possible to obtain a highly comprehensive representation of the silver fir's transcriptome. The assembled transcriptome shows a completeness value of 94.8%, with 539,313 assembled transcripts, of which approximately 30% were functionally annotated. It constitutes the highest quality transcriptome available to date for A. alba, providing a valuable tool for further studies involving this species.
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García-García et al. (2026) studied this question.
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