Activation of NF-κB signaling in tissue-resident memory T cells promotes recurrent psoriasis in mice

Background Recurrence triggered by immunological memory is a critical challenge in the treatment of psoriasis. Tissue-resident memory T (Trm) cells are the primary pacemakers in recurrent psoriasiform dermatitis following stimulation and infection by previous pathogens. However, the mechanisms underlying Trm cell activation remain unclear. Methods In this study, imiquimod-induced recurrent psoriatic mice were established to characterize the phenotypes of different Trm cell subsets. CD8 + /CD4 + Tcm cell injection and NF-κB inhibitor or agonist treatment were then used to investigate the role and mechanisms of Trm cells in recurrent psoriasis. Finally, CD8 + T cells and keratinocyte co-culture systems were established to investigate the effects of activating NF-κB activation in Trm cells. Results Mice displayed severe psoriatic dermatitis after repeated imiquimod treatment. The CD8 + Trm cells, but not CD4 + Trm cells, were elevated in the skin of recurrent psoriatic mice. Injection of CD8 + Tcm cells injection elicited more severe psoriatic symptoms than imiquimod treatment alone, indicating that CD8 + Trm cells are critical participants in psoriatic recurrence. Phosphorylation of NF-kB p65 (RELA), p-IKKa, p-RelB and NF-kB p100/p52 was enhanced in the skin of imiquimod-induced recurrent psoriatic mice. NF-κB inhibitor/agonist treatment significantly suppressed or restored the dermatitis severity and CD8 + Trm cell levels in recurrent psoriatic mice. Meanwhile, NF-κB inhibition also restored the expression of DLAT in Trm cells. Finally, NF-κB inhibitors directly suppressed Trm cell activation and inflammation of Trm cells in vitro . Conclusion Together, these findings suggest that canonical and non-canonical NF-κB signaling directly activates Trm cell differentiation, inhibits cellular cuproptosis, and promotes recurrent psoriasis.