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The aim is to explore adaptive mechanisms for drought tolerance in different soybean genotypes.

Integrated physiological, biochemical, and molecular analysis of drought tolerance in soybean cultivars

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Demonstrates the mechanisms of drought tolerance in soybean, indicating potential for breeding resilient cultivars.

The aim is to explore adaptive mechanisms for drought tolerance in different soybean genotypes.
Conducted pot experiments under controlled conditions with seven soybean genotypes.
Measured physiological traits such as relative water content, chlorophyll content, and photosynthetic rate.
Evaluated biochemical indicators including proline, glycine betaine, and antioxidant enzyme activities.
Performed qRT-PCR for gene expression analysis of drought-responsive genes.
Applied multivariate analyses to investigate correlations among traits.
Tolerant genotypes maintained higher relative water content (>80%) and chlorophyll (≥1.73 g g −1 FW) under drought conditions.
Enhanced accumulation of osmolytes like proline (up to 47.5 µg g −1 FW) and glycine betaine (up to 157 µg g −1 FW) in tolerant genotypes.
Significantly higher antioxidant enzyme activities in tolerant genotypes compared to susceptible ones.
Higher expression of stress-responsive genes, with GmP5CS showing a ~3.8-fold increase in tolerant genotypes.

Fahad Alghabari (2026) studied this question.

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