Key result
Angiotensin II infusion alters 324 circular RNAs during abdominal aortic aneurysm formation in mice.
Why the study?
Effective pharmacological treatments for abdominal aortic aneurysm (AAA) are lacking, and the molecular regulatory mechanisms involving circRNAs in AAA remain unclear.
Does Angiotensin II infusion alter the expression profile of circular RNAs in the abdominal aorta of apoE-/- mice?
Does Angiotensin II infusion alter the expression profile of circular RNAs in the abdominal aorta of apoE-/- mice?
p-value: p=<0.05
Angiotensin II-induced abdominal aortic aneurysm in mice is associated with significant differential expression of circRNAs, suggesting the circRNA-miRNA-mRNA axis as a potential regulatory mechanism in AAA pathogenesis.
BACKGROUND: Abdominal aortic aneurysm (AAA) is a cardiovascular condition characterized by the abnormal dilation of the abdominal aorta. OBJECTIVES: A circular RNA (circRNA) microarray was utilized to identify differentially expressed circRNAs in angiotensin II (Ang II)-stimulated AAA mice. MATERIAL AND METHODS: Male apolipoprotein E-deficient (apoE-/-) mice were randomly assigned to 2 groups and subjected to 28 days of infusion with either Ang II or saline. At the end of the experiment, the mice were euthanized via exsanguination under anesthesia. The periadventitial tissues were carefully removed from the aortic wall to measure the maximal external diameter of the suprarenal aorta, and then stored for further analysis. Samples from both the control and AAA groups were used for circRNA expression profiling. The R package Bioconductor was employed to perform Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Arraystar's proprietary miRNA target prediction software, integrating miRanda and TargetScan, was used to predict the circRNA/miRNA interactions. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was employed to confirm the reliability of the microarray results. RESULTS: A total of 13,103 circRNAs were detected. Compared to the control group, 90 circRNAs were upregulated and 234 were downregulated in the Ang II-induced AAA group. Gene Ontology analysis indicated that the target genes associated with the differentially expressed circRNAs were involved in a variety of biological processes. The KEGG pathway analysis revealed that the differentially expressed circRNAs influenced several critical pathways, including the MAPK signaling pathway, insulin signaling pathway, Ras signaling pathway, and autophagy. The results of RT-qPCR showed that the expression levels of circRNA_30395, circRNA_30398 and circRNA_012594 were significantly increased in AAA, while circRNA_006097 and circRNA_009932 were notably decreased. The top 5 miRNAs related to each validated circRNA were identified through bioinformatic analysis. Among these differentially expressed circRNAs, miR-136-5p was predicted to be the target gene of circRNA_30398 with high probability. CONCLUSIONS: The differential expression of various circRNAs identified in AAA suggests that the circRNA-miRNA-mRNA axis may serve as a potential molecular regulatory mechanism for AAA.
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Lou et al. (2026) studied Abdominal aortic aneurysm (n=30). Angiotensin II vs. Normal saline was evaluated on Differentially expressed circRNAs (p=<0.05). Angiotensin II infusion in apoE-/- mice induced abdominal aortic aneurysm and resulted in 324 significantly differentially expressed circRNAs (90 upregulated, 234 downregulated) compared to saline.
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