The aim is to create a new fluorescent probe for effective DNA detection and visualization within cells.
Designed a fluorescent probe by linking pyrene to a naphthalimide-imidazole unit via a benzene spacer.
Examined the functionality based on π–π stacking and excimer formation for enhanced fluorescence properties.
Conducted tests for intracellular visualization of DNA using the developed probe.
The probe exhibited strong fluorescence signaling upon binding to DNA.
Demonstrated effective cellular uptake, allowing for visualization of DNA within live cells.
Highlighted potential applications in biological imaging and detection of nucleic acids.
Abstract
A novel fluorescent probe L for DNA detection was designed by linking a pyrene fluorophore to a naphthalimide-imidazole unit via a benzene spacer. The probe operates through a π–π stacking-driven...