A pyrene-derived fluorescence probe based on π-π stacking and excimer formation for DNA detection and intracellular visualization

Songtao Cai; Chuanyan Zhou; Caixia Xue; Yanfang Huo; Chang Liu; Yingying Jing; Yan Fan; Yanxia Zhang; Chuan-Min Qi

doi:10.1039/d5ay02071a

January 1, 2026Analytical Methods

A pyrene-derived fluorescence probe based on π-π stacking and excimer formation for DNA detection and intracellular visualization

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Key Points

The aim is to create a new fluorescent probe for effective DNA detection and visualization within cells.
Designed a fluorescent probe by linking pyrene to a naphthalimide-imidazole unit via a benzene spacer.
Examined the functionality based on π–π stacking and excimer formation for enhanced fluorescence properties.
Conducted tests for intracellular visualization of DNA using the developed probe.
The probe exhibited strong fluorescence signaling upon binding to DNA.
Demonstrated effective cellular uptake, allowing for visualization of DNA within live cells.
Highlighted potential applications in biological imaging and detection of nucleic acids.

Implication

Novel fluorescent probe detects DNA in cells, suggesting new methods for intracellular visualization.

Abstract

A novel fluorescent probe L for DNA detection was designed by linking a pyrene fluorophore to a naphthalimide-imidazole unit via a benzene spacer. The probe operates through a π–π stacking-driven...

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Cite This Study

Cai et al. (2026) studied this question.

synapsesocial.com/papers/698d6e055be6419ac0d535ee https://doi.org/10.1039/d5ay02071a

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A pyrene-derived fluorescence probe based on π-π stacking and excimer formation for DNA detection and intracellular visualization

Key Points

Implication

Abstract

Discussion

Cite This Study

A pyrene-derived fluorescence probe based on π-π stacking and excimer formation for DNA detection and intracellular visualization

Key Points

Implication

Abstract

Discussion

Cite This Study

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