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February 12, 2026Applied Sciences3 citationsOpen Access

Microwave-Assisted Extraction of Bioactive Compounds and Chemical Characterisation of Coffee By-Products and Coffee

AGAntonela Ninčević GrassinoVKVeronika KovačSPSandra Pedišić

Key Points

  • This research aims to explore the extraction of bioactive compounds from coffee and its by-products using microwave-assisted extraction.
  • Utilized microwave-assisted extraction for bioactive compound recovery from coffee residues.
  • Conducted principal component analysis to assess compound variations between coffee types and by-products.
  • Employed a linear mixed-effects model to analyze the effects of extraction temperature and ethanol volume on compound yields.
  • The extraction at 70% ethanol and 70 °C yielded the highest amounts of bioactive compounds.
  • Total fibre levels ranged from 20.7% to 27.8%, and total fat content varied between 1.5% and 12.4%.
  • Fatty acid analysis revealed significant amounts of oleic, palmitic, and linoleic acids, confirmed by FTIR spectroscopy.

Abstract

This study reports the effective reuse of coffee residues from roasting and brewing processes using microwave-assisted extraction (MAE) of bioactive compounds. Principal Component Analysis (PCA) showed a significant impact (p < 0.001) on phenolic acid and alkaloid amounts among coffee (green and roasted) and its by-products (silver skin and spent grounds). Furthermore, a linear mixed-effects model identified that, apart from sample type, extraction temperature (50 and 70 °C) and ethanol volume fractions (50 and 70%, v/v) considerably influenced the quantities of bioactive compounds. The best conditions were achieved using 70% (v/v) ethanol at 70 °C. In addition to caffeine and chlorogenic acid, which were found in the highest amounts, the coffee and by-product samples contained high levels of total fibre (20.7–27.8%) and total fat (1.5–12.4%). Fatty acid analysis showed a dominance of oleic acid (7.55–12.7%), palmitic acid (19.3–40.3%), and linoleic acid (15.6–41.8%), with their saturated and unsaturated nature confirmed by Fourier Transform Infrared (FTIR) Spectroscopy. The reported data highlight the potential of coffee by-products as high-value sources for bioactive compound recovery.

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Cite This Study

Grassino et al. (2026) studied this question.

synapsesocial.com/papers/698d6f5f5be6419ac0d55324https://doi.org/10.3390/app16041794
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