Key result
Simultaneous MYO1 gene expression profiling differentiated haematologic malignancy cell lines by showing decreased MYO1B, MYO1C, MYO1D, MYO1E and increased MYO1G and MYO1F expression compared to other cells.
Why the study?
Most studies examine class I myosins individually or in small groups, ignoring potential redundancy and functional substitution, which hinders assessing their biomarker potential and mechanistic role in diseases.
Effect estimate: Significant differential expression pattern distinguishing haematologic malignancies with decreased expression of MYO1B, MYO1C, MYO1D, MYO1E and increased expression of MYO1G and MYO1F compared to other cell lines
p-value: p=Various MYO1 genes showed significance with p<0.05 to p<0.001 according to Mann–Whitney U test with Benjamini–Hochberg correction
Developed a qPCR system and utilized RNA-seq to clarify the restricted expression patterns of all eight human MYO1 genes across various cell types, including haematopoietic lines.
Class I myosins form an abundant group of single-headed motor proteins that interact with actin and membrane phospholipids and are employed in a variety of cell processes like cell shape remodeling, motility and invasion, exocytosis, and endocytosis. The large number of class members and their uneven distribution in human cells and tissues complicate their study. Furthermore, this hinders the assessment of their biomarker potential and mechanistic role in oncogenesis and other diseases. Despite the emerging data on their mutations and altered abundance in a number of tumors and other diseases, most studies examine class I myosins individually or in groups of several proteins. Thus, the question of their redundancy and possible functional substitution is ignored. In this work, we simultaneously assess all eight human MYO1 genes on cell lines of different origins by RNA-seq re-analysis. We also report a qPCR system for human myosin I screening and test it on a set of 35 continuous cell lines widely used in biomedicine and fundamental research. These data clarify the restricted expression patterns of MYO1 genes in various human cell types and allow to assess the pattern specific of haematopoietic cell lines.
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Golysheva et al. (2026) studied Human malignant and non-malignant cell lines including haematologic malignancies and various tissue origins (n=66). Simultaneous assessment of all eight human MYO1 genes expression vs. Comparison between malignant and non-malignant cell lines of various origins was evaluated on Expression pattern of all eight human MYO1 genes in human cell lines distinguishing haematologic malignancies from others (Significant differential expression pattern distinguishing haematologic malignancies with decreased expression of MYO1B, MYO1C, MYO1D, MYO1E and increased expression of MYO1G and MYO1F compared to other cell lines, p=Various MYO1 genes showed significance with p<0.05 to p<0.001 according to Mann–Whitney U test with Benjamini–Hochberg correction). Simultaneous MYO1 gene expression profiling differentiated haematologic malignancy cell lines by showing decreased MYO1B, MYO1C, MYO1D, MYO1E and increased MYO1G and MYO1F expression compared to other cells.
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