Influence of the Gut Microbiome on Body Composition and Metabolic Risk in Indian Children with Type 1 Diabetes Mellitus (T1dm)

A BSTRACT Introduction: The study aims to explore the gut flora presentation among Type 1 DM children with and without metabolic risks (MR) at a tertiary care center. Materials and Methods: A cross-sectional, observational study was conducted on children with T1DM (n = 68, mean-age = 12.9 years, disease-duration = 5.5 years, average HbA1c = 9.8%) divided into two groups based on with (n = 35) and without (n = 33) metabolic risk factors. Clinical-history, anthropometry, biochemical investigations, and body composition analysis was performed. Stool samples were collected; DNA extraction and targeted 16srRNA gene amplicon sequencing was performed. Results: Significant differences were observed in the relative abundance of gut microbiome at the family and genera level in children with metabolic risk as compared to without metabolic risk. Conclusion: Gut microbiome significantly differs in children with or without MR among T1DM cases.