What does this research mean for the field?

Five hub genes (MMP9, KLRB1, S100A8, S100A12, and MMP8) are identified as strong diagnostic biomarkers for sepsis, correlating with immune cell infiltration and linked to critical immunological pathways. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to explore the molecular mechanisms and immune profiles of sepsis to enhance diagnostic strategies.

February 19, 2026International Journal of Clinical PracticeOpen Access

Investigation of the Molecular Mechanisms Underlying Sepsis Through Integration of Single‐Cell Sequencing and Transcriptome Sequencing

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Authors

WRWei RenShanghai Jiao Tong University MLMin LiWenzhou Medical University ZWZhi-Min WangWenzhou Medical University

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Implication

Investigation reveals immune profiles and potential biomarkers in sepsis, suggesting new diagnostic approaches.

Key Points

This research aims to explore the molecular mechanisms and immune profiles of sepsis to enhance diagnostic strategies.
Analyzed single-cell RNA sequencing data to identify subtype-specific marker genes.
Integrated multiple GEO datasets for differential expression analysis using the limma package.
Applied feature selection algorithms for hub gene identification and created diagnostic models.
Validated model efficacy through ROC analysis and explored relationships between hub genes and immune cell infiltration.
Identified 493 subtype-specific marker genes and 19 clusters across six immune cell types.
Revealed 140 upregulated and 98 downregulated genes from integrated RNA-seq analysis.
The XGB diagnostic model achieved an AUC of 0.884, indicating strong predictive capability.
Five hub genes were correlated with immune cells and linked to significant immunological pathways.

Cite This Study

Ren et al. (2026) studied this question.

synapsesocial.com/papers/6996a818ecb39a600b3ee7cb https://doi.org/10.1155/ijcp/8359255

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Investigation of the Molecular Mechanisms Underlying Sepsis Through Integration of Single‐Cell Sequencing and Transcriptome Sequencing

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Implication

Key Points

Cite This Study

Investigation of the Molecular Mechanisms Underlying Sepsis Through Integration of Single‐Cell Sequencing and Transcriptome Sequencing

Discussion

Member takes

Implication

Key Points

Cite This Study

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