Effects of New Cellular Treatment Factor and Human Exosome Injection on Intact Skin Regeneration in a Randomized In Vivo Study

Background: Enhancing intact skin regeneration is a growing focus in dermatology and aesthetics. Human derived exosomes and mesotherapy formulations such as New Cellular Treatment Factor (NCTF) have shown regenerative potential, yet their combined effects on healthy skin remain unstudied. This study evaluated NCTF, two exosome products, and their combinations on markers of skin regeneration. Methods: A randomized, within-animal controlled study was conducted in 144 adult male Syrian golden hamsters. Each animal received 6 intradermal injections on the dorsum: saline control, CellExosome, ASCE+ Exosome, NCTF, NCTF + CellExosome, or NCTF + ASCE+ Exosome. Skin biopsies were obtained at baseline and on days 3, 7, and 14. Histological assessments (hematoxylin and eosin, Van Gieson, Masson trichrome) evaluated dermal structure and collagen deposition. CD34 immunohistochemistry quantified microvascular density. Results: By day 14, collagen synthesis and vascular proliferation were significantly greater in all active treatment groups compared with the control ( P < 0.0001). The NCTF + CellExosome combination produced the highest improvements in collagen density and microvascularity, followed by NCTF + ASCE+ Exosome, NCTF alone, ASCE+ Exosome, and CellExosome. These findings indicate synergistic biological effects when NCTF is combined with exosomes. Conclusions: NCTF and human-derived exosomes enhance dermal structural regeneration and angiogenesis in intact skin. Combination therapy, particularly NCTF + CellExosome, provides the strongest regenerative response, supporting further investigation for clinical aesthetic applications.