What question did this study set out to answer?

The aim is to assess whether enhancing IKZF1 expression can improve CD19 levels, thereby increasing CAR T-cell targeting of MCL.

February 20, 2026Cancer Immunology Research

Abstract C009: Transcriptional regulation of CD19 by IKZF1 enhances CAR T-Cell immune targeting in mantle cell lymphoma

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Authors

ASAnya K. SondhiSylvester Comprehensive Cancer Center TTTulasigeri M. TotigerUniversity of Miami SIStanzin IdgaNYU Langone Health

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Implication

Demonstrates enhanced CAR T-cell killing in mantle cell lymphoma by modulating CD19 levels via IKZF1 modification, suggesting potential therapeutic strategies.

Key Points

The aim is to assess whether enhancing IKZF1 expression can improve CD19 levels, thereby increasing CAR T-cell targeting of MCL.
Utilized lentiviral vectors for IKZF1 overexpression in JeKo-1 and Mino MCL cell lines.
Inhibited CK2α with silmitasertib to increase endogenous IKZF1 stability.
Measured CD19 surface density using flow cytometry and CD19 transcripts by RT-qPCR.
Evaluated CAR T-cell activity in co-cultures, measuring target cell viability and cytokine secretion.
Established xenografts in NSG mice for in vivo CAR T-cell analysis, monitoring tumor burden.
IKZF1 overexpression increased CD19 mRNA and protein levels by approximately 2.5-fold.
CK2α inhibition also elevated IKZF1 and CD19 expression levels.
CAR T-cell killing was enhanced, with target cell viability dropping to 41.5% with IKZF1 modulation at E:T 1:1.
Elevated IFN-γ, TNF, and IL-2 secretion from CAR T-cells, all statistically significant (p < 0.001).
In vivo studies confirmed rapid CAR T-cell efficacy correlated with higher CD19 expression.

Cite This Study

Sondhi et al. (2026) studied this question.

synapsesocial.com/papers/6997fa6dad1d9b11b3453ab4 https://doi.org/10.1158/2326-6074.io2026-c009

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Implication

Demonstrates enhanced CAR T-cell killing in mantle cell lymphoma by modulating CD19 levels via IKZF1 modification, suggesting potential therapeutic strategies.

Abstract C009: Transcriptional regulation of CD19 by IKZF1 enhances CAR T-Cell immune targeting in mantle cell lymphoma

Discussion

Member takes

Implication

Key Points

Cite This Study

Abstract C009: Transcriptional regulation of CD19 by IKZF1 enhances CAR T-Cell immune targeting in mantle cell lymphoma

Discussion

Member takes

Implication

Key Points

Cite This Study

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